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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RGN/SMP30 Polyclonal antibody (17947-1-AP)

    WB analysis using 17947-1-AP (same clone as 17947-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using RGN/SMP30 Polyclonal antibody (17947-1-AP)

    WB analysis of mouse small intestine using 17947-1-AP (same clone as 17947-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using RGN/SMP30 Polyclonal antibody (17947-1-AP)

    IP experiment of mouse liver using 17947-1-AP (same clone as 17947-1-PBS)

    IP result of anti-RGN/SMP30 (IP:17947-1-AP, 4ug; Detection:17947-1-AP 1:15000) with mouse liver tissue lysate 3040 ug. This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using RGN/SMP30 Polyclonal antibody (17947-1-AP)

    IHC staining of rat kidney using 17947-1-AP (same clone as 17947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using RGN/SMP30 Polyclonal antibody (17947-1-AP)

    IHC staining of rat kidney using 17947-1-AP (same clone as 17947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using RGN/SMP30 Polyclonal antibody (17947-1-AP)

    IHC staining of human hepatocirrhosis using 17947-1-AP (same clone as 17947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using RGN/SMP30 Polyclonal antibody (17947-1-AP)

    IHC staining of human hepatocirrhosis using 17947-1-AP (same clone as 17947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 17947-1-AP (RGN/SMP30 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RGN/SMP30 Polyclonal antibody (17947-1-AP)

    IHC staining of human kidney using 17947-1-AP (same clone as 17947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 17947-1-AP (RGN/SMP30 antibody at dilution of 1:50. This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RGN/SMP30 Polyclonal antibody (17947-1-AP)

    IHC staining of human kidney using 17947-1-AP (same clone as 17947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 17947-1-AP (RGN/SMP30 antibody at dilution of 1:50. This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using RGN/SMP30 Polyclonal antibody (17947-1-AP)

    IHC staining of human liver using 17947-1-AP (same clone as 17947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 17947-1-AP (RGN/SMP30 antibody at dilution of 1:50. This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using RGN/SMP30 Polyclonal antibody (17947-1-AP)

    IHC staining of human liver using 17947-1-AP (same clone as 17947-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 17947-1-AP (RGN/SMP30 antibody at dilution of 1:50. This data was developed using the same antibody clone with 17947-1-PBS in a different storage buffer formulation.

RGN/SMP30 Polyclonal antibody, PBS Only

RGN/SMP30 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17947-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17947-1-PBS targets RGN/SMP30 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12423

Product name: Recombinant human RGN,SMP30 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-299 aa of BC073173

Sequence: MSSIKIECVLPENCRCGESPVWEEVSNSLLFVDIPAKKVCRWDSFTKQVQRVTMDAPVSSVALRQSGGYVATIGTKFCALNWKEQSAVVLATVDNDKKNNRFNDGKVDPAGRYFAGTMAEETAPAVLERHQGALYSLFPDHHVKKYFDQVDISNGLDWSLDHKIFYYIDSLSYSVDAFDYDLQTGQISNRRSVYKLEKEEQIPDGMCIDAEGKLWVACYNGGRVIRLDPVTGKRLQTVKLPVDKTTSCCFGGKNYSEMYVTCARDGMDPEGLLRQPEAGGIFKITGLGVKGIAPYSYAG

相同性解析による交差性が予測される生物種
Full Name regucalcin (senescence marker protein-30)
Calculated molecular weight 299 aa, 33 kDa
Observed molecular weight 33 kDa, 25 kDa
GenBank accession numberBC073173
Gene Symbol RGN/SMP30
Gene ID (NCBI) 9104
RRIDAB_10644486
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15493
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Regucalcin (RGN), also known as SMP30, is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. SMP30 is preferentially localized in hepatocytes and renal proximal tubular epithelial cells. It is known to be related to aging, hepatocyte proliferation and tumorigenesis. It may be useful for HCC serologic screening, especially for the patients with AFP Negative. RGN encodes two isoforms: 33 kDa and 25 kDa.