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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using REST Polyclonal antibody (22242-1-AP)

    WB analysis of Raji using 22242-1-AP (same clone as 22242-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 22242-1-AP (REST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using REST Polyclonal antibody (22242-1-AP)

    WB analysis of HEK-293 using 22242-1-AP (same clone as 22242-1-PBS)

    WB result of REST antibody (22242-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REST transfected HEK-293 cells. This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using REST Polyclonal antibody (22242-1-AP)

    IP experiment of HeLa using 22242-1-AP (same clone as 22242-1-PBS)

    IP result of anti-REST (IP:22242-1-AP, 4ug; Detection:22242-1-AP 1:15000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using REST Polyclonal antibody (22242-1-AP)

    IHC staining of human breast cancer using 22242-1-AP (same clone as 22242-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using REST Polyclonal antibody (22242-1-AP)

    IHC staining of human breast cancer using 22242-1-AP (same clone as 22242-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

REST Polyclonal antibody, PBS Only

REST Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 22242-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

NRSF, X2 box repressor, XBR

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22242-1-PBS targets REST in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17707

Product name: Recombinant human REST protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 748-1097 aa of BC132859

Sequence: ELSPPMEVVQKEPVKIELSPPIEVVQKEPVQMELSPPMGVVQKEPAQREPPPPREPPLHMEPISKKPPLRKDKKEKSNMQSERARKEQVLIEVGLVPVKDSWLLKESVSTEDLSPPSPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVGAEEADESLPGLAANINESTHISSSGQNLNTPEGETLNGKHQTDSIVCEMKMDTDQNTRENLTGINSTVEEPVSPMLPPSAVEEREAVSKTALASPPATMAANESQEIDEDEGIHSHEGSDLSDNMSEGSDDSGLHGARPVPQESSRKNAKEALAVKAAKGDFVCIFCDRSFRKGKDYSKHLNRHLVNVYYLEEAAQGQE

相同性解析による交差性が予測される生物種
Full Name RE1-silencing transcription factor
Calculated molecular weight 1097 aa, 122 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC132859
Gene Symbol REST
Gene ID (NCBI) 5978
RRIDAB_2879044
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13127
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).

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