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REG3A Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.312-40 ng/mL

Sandwich ELISA: 0.313-20 ng/mL

Cat no : MP02800-1

Print datasheet

Synonyms

Hepatointestinal pancreatic protein, HIP, HIP/PAP, Pancreatitis-associated protein 1, Reg III-alpha

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02800-1, Human REG3A Recombinant Matched Antibody Pair - PBS only. 86976-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2893. 86976-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

    Sandwich ELISA standard curve of MP02800-1

    Sandwich ELISA standard curve of MP02800-1, Human REG3A Recombinant Matched Antibody Pair - PBS only. 86976-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2893. 86976-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Plasma of eight individual healthy human donors was measured. The REG3A concentration of detected samples was determined to be 1.62 ng/mL with a range of 0.15-4.64 ng/mL.

    Sandwich ELISA sample test of MP02800-1

    Plasma of eight individual healthy human donors was measured. The REG3A concentration of detected samples was determined to be 1.62 ng/mL with a range of 0.15-4.64 ng/mL.

  • Serum of six individual healthy human donors was measured. The REG3A concentration of detected samples was determined to be 1.66 ng/mL with a range of 0.10-8.90 ng/mL.

    Sandwich ELISA sample test of MP02800-1

    Serum of six individual healthy human donors was measured. The REG3A concentration of detected samples was determined to be 1.66 ng/mL with a range of 0.10-8.90 ng/mL.

  • Cytometric bead array standard curve of MP02800-1, REG3A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86976-2-PBS. Detection antibody: 86976-1-PBS. Standard: Eg2893. Range: 0.312-40 ng/mL.

    Cytometric bead array standard curve of MP02800-1

    Cytometric bead array standard curve of MP02800-1, REG3A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86976-2-PBS. Detection antibody: 86976-1-PBS. Standard: Eg2893. Range: 0.312-40 ng/mL.

  • The mean REG3A concentration was determined to be 58.3 ng/mL in human plasma.

    Cytometric bead array sample test of MP02800-1

    The mean REG3A concentration was determined to be 58.3 ng/mL in human plasma.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02800-1 targets REG3A in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.312-40 ng/mL (Cytometric Bead Array); 0.313-20 ng/mL (Sandwich ELISA);

Capture antibody: REG3A Recombinant monoclonal antibody, PBS Only (Capture) 86976-2-PBS (251991E11). 100 μg. Concentration 1 mg/ml.

Detection antibody: REG3A Recombinant monoclonal antibody, PBS Only (Detector) 86976-1-PBS (251991G5). 100 μg. Concentration 1 mg/ml.

Alternative REG3A matched antibody pairs: MP50201-1

Matched pair protein standard: Eg2893

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 86976-2-PBS
Tested Reactivity human
Immunogen REG3A fusion protein Eg2893
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name regenerating islet-derived 3 alpha
Synonyms
GenBank accession numberNM_002580.2
Gene symbol REG3A
UniprotQ06141
Gene ID (NCBI) 5068
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 86976-1-PBS
Tested Reactivity human
Immunogen REG3A fusion protein Eg2893
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name regenerating islet-derived 3 alpha
Synonyms
GenBank accession numberNM_002580.2
Gene symbol REG3A
UniprotQ06141
Gene ID (NCBI) 5068
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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