• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Purchase

Recombinant Human REG3A protein (rFc Tag)(HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2893

Validation Data Gallery

  • Purity of Recombinant Human REG3A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human REG3A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human REG3A was greater than 90% as determined by SEC-HPLC.

    The purity of Human REG3A was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human REG3A protein Glu27-Asp175 (Accession# Q06141) with a rabbit IgG Fc tag at the C-terminus.
GeneID 5068
Accession Q06141
PredictedSize 45.8 kDa
SDS-PAGE 40-47 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Regenerating islet-derived protein 3-alpha (REG3A), also known as hepatocarcinoma-intestine pancreas/pancreatic associated protein or HIP/PAP, is a secreted 16 kDa carbohydrate-binding protein that functions as an AMP against Gram-positive bacteria. REG3A is reported to be associated with diseases in a variety of organs and tissues such as pancreas, gastrointestinal tract, liver, heart, and skin. REG3A expression is highly upregulated in inflamed pancreas and gastrointestinal tract. The main biological functions of REG3A are related to cell proliferation, regeneration, resistance to cell death, and tissue repair after inflammatory injury.

References:

1.Guo J. et al. (2021) Mol Cells. 44(9):647-657.
2.Wang L. et al. (2021) Bioengineered. 12(1):7644-7655.
3.Gonzalez P. et al. (2023) Commun Biol. 6(1):269.
4.Yu L. et al. (2023) Biomed Pharmacother. 169:115922.