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RDBP Recombinant antibody

RDBP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85945-1-RR
Clone No.250420G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

NELFE, NELF E, NELF-E, RD, RNA-binding protein RD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, Calu-3 cells, mouse brain tissue, rat brain tissue, HEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:7500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85945-1-RR targets RDBP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RDBP fusion protein Ag1186 相同性解析による交差性が予測される生物種
Full Name RD RNA binding protein
Calculated molecular weight 37 kDa, 43 kDa
Observed molecular weight42 kDa
GenBank accession numberBC011600
Gene Symbol NELFE
Gene ID (NCBI) 7936
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18615
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

Protocols

Product Specific Protocols
WB protocol for RDBP antibody 85945-1-RRDownload protocol
IF protocol for RDBP antibody 85945-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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