Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, Calu-3 cells, mouse brain tissue, rat brain tissue, HEK-293 cells |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2500-1:7500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85945-1-RR targets RDBP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | RDBP fusion protein Ag1186 相同性解析による交差性が予測される生物種 |
Full Name | RD RNA binding protein |
Calculated molecular weight | 37 kDa, 43 kDa |
Observed molecular weight | 42 kDa |
GenBank accession number | BC011600 |
Gene Symbol | NELFE |
Gene ID (NCBI) | 7936 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P18615 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.
Protocols
Product Specific Protocols | |
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WB protocol for RDBP antibody 85945-1-RR | Download protocol |
IF protocol for RDBP antibody 85945-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |