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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RDBP Recombinant antibody (85945-1-RR)

    WB analysis using 85945-1-RR (same clone as 85945-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RDBP Recombinant antibody (85945-1-RR)

    WB analysis using 85945-1-RR (same clone as 85945-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RDBP Recombinant antibody (85945-1-RR)

    IF Staining of HeLa using 85945-1-RR (same clone as 85945-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RDBP antibody (85945-1-RR, Clone: 250420G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

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RDBP Recombinant antibody, PBS Only

RDBP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 85945-1-PBS
Clone No.250420G2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

NELFE, NELF E, NELF-E, RD, RNA-binding protein RD

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85945-1-PBS targets RDBP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RDBP fusion protein Ag1186 相同性解析による交差性が予測される生物種
Full Name RD RNA binding protein
Calculated molecular weight 37 kDa, 43 kDa
Observed molecular weight42 kDa
GenBank accession numberBC011600
Gene Symbol NELFE
Gene ID (NCBI) 7936
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18615
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.