Validation Data Gallery
Tested Applications
| Positive WB detected in | rat liver tissue, HeLa cells, HEK-293 cells, U-251 cells, mouse liver tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84956-4-RR targets RCN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0247 Product name: Recombinant human RCN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-209 aa of BC004892 Sequence: GKAEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLE 相同性解析による交差性が予測される生物種 |
| Full Name | reticulocalbin 2, EF-hand calcium binding domain |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC004892 |
| Gene Symbol | RCN2 |
| Gene ID (NCBI) | 5955 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14257 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Reticulocalbin-2 (RCN2) is also named as endoplasmic reticulum Ca2+-binding protein of 55 kDa (ERC55) and E6-binding protein (E6BP). It is strictly located in endoplasmic reticulum (PMID: 34094681). RCN2 is a low-affinity Ca2+ binding protein localized to the secretory pathway of mammalian cells, belongs to a member of the EF-hand Ca2+ binding protein family, which participate in the secretory process, chaperone activity, signal transduction as well as participation in a large variety of disease processes (PMID: 19260022). As for cancer research, several studies showed that RCN2 was related to tumor progression, such as mammary cancer, cervical cancer, neck squamous cell carcinoma and colorectal cancer (PMID: 16351756, PMID: 32422974). RCN2 played a pivotal role in HCC pathogenesis through regulating activation of the epidermal growth factor receptor (EGFR) pathway and modulating epidermal growth factor receptor EGFR dimerization and internalization (PMID: 28745317).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RCN2 antibody 84956-4-RR | Download protocol |
| IHC protocol for RCN2 antibody 84956-4-RR | Download protocol |
| WB protocol for RCN2 antibody 84956-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |