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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RCN2 Recombinant antibody (84956-4-RR)

    WB analysis using 84956-4-RR (same clone as 84956-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84956-4-RR (RCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RCN2 Recombinant antibody (84956-4-RR)

    WB analysis using 84956-4-RR (same clone as 84956-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84956-4-RR (RCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RCN2 Recombinant antibody (84956-4-RR)

    IHC staining of mouse testis using 84956-4-RR (same clone as 84956-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84956-4-RR (RCN2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RCN2 Recombinant antibody (84956-4-RR)

    IF Staining of HeLa using 84956-4-RR (same clone as 84956-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RCN2 Recombinant antibody (84956-4-RR)

    IF Staining of HepG2 using 84956-4-RR (same clone as 84956-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

RCN2 Recombinant monoclonal antibody, PBS Only

RCN2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84956-4-PBS
Clone No.242612A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242612A4, Calcium binding protein ERC 55, Calcium-binding protein ERC-55, E6 binding protein, E6-binding protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84956-4-PBS targets RCN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0247

Product name: Recombinant human RCN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-209 aa of BC004892

Sequence: GKAEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLE

相同性解析による交差性が予測される生物種
Full Name reticulocalbin 2, EF-hand calcium binding domain
Calculated molecular weight 37 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC004892
Gene Symbol RCN2
Gene ID (NCBI) 5955
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14257
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Reticulocalbin-2 (RCN2) is also named as endoplasmic reticulum Ca2+-binding protein of 55 kDa (ERC55) and E6-binding protein (E6BP). It is strictly located in endoplasmic reticulum (PMID: 34094681). RCN2 is a low-affinity Ca2+ binding protein localized to the secretory pathway of mammalian cells, belongs to a member of the EF-hand Ca2+ binding protein family, which participate in the secretory process, chaperone activity, signal transduction as well as participation in a large variety of disease processes (PMID: 19260022). As for cancer research, several studies showed that RCN2 was related to tumor progression, such as mammary cancer, cervical cancer, neck squamous cell carcinoma and colorectal cancer (PMID: 16351756, PMID: 32422974). RCN2 played a pivotal role in HCC pathogenesis through regulating activation of the epidermal growth factor receptor (EGFR) pathway and modulating epidermal growth factor receptor EGFR dimerization and internalization (PMID: 28745317).

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