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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RCN2 Polyclonal antibody (10193-2-AP)

    WB analysis using 10193-2-AP (same clone as 10193-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RCN2 Polyclonal antibody (10193-2-AP)

    WB analysis of human brain using 10193-2-AP (same clone as 10193-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RCN2 Polyclonal antibody (10193-2-AP)

    WB analysis using 10193-2-AP (same clone as 10193-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using RCN2 Polyclonal antibody (10193-2-AP)

    WB analysis of mouse lung using 10193-2-AP (same clone as 10193-2-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 10193-2-AP (RCN2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using RCN2 Polyclonal antibody (10193-2-AP)

    IP experiment of mouse lung using 10193-2-AP (same clone as 10193-2-PBS)

    IP result of anti-RCN2 (IP:10193-2-AP, 4ug; Detection:10193-2-AP 1:400) with mouse lung tissue lysate 2800ug. This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RCN2 Polyclonal antibody (10193-2-AP)

    IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RCN2 Polyclonal antibody (10193-2-AP)

    IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RCN2 Polyclonal antibody (10193-2-AP)

    IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RCN2 Polyclonal antibody (10193-2-AP)

    IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RCN2 Polyclonal antibody (10193-2-AP)

    IHC staining of human gliomas using 10193-2-AP (same clone as 10193-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RCN2 Polyclonal antibody (10193-2-AP)

    IHC staining of human testis using 10193-2-AP (same clone as 10193-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RCN2 Polyclonal antibody (10193-2-AP)

    IHC staining of human testis using 10193-2-AP (same clone as 10193-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RCN2 Polyclonal antibody (10193-2-AP)

    IHC staining of human testis using 10193-2-AP (same clone as 10193-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RCN2 Polyclonal antibody (10193-2-AP)

    IHC staining of human testis using 10193-2-AP (same clone as 10193-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 10193-2-AP (RCN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RCN2 Polyclonal antibody (10193-2-AP)

    IF Staining of HeLa using 10193-2-AP (same clone as 10193-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RCN2 Polyclonal antibody (10193-2-AP)

    IF Staining of HepG2 using 10193-2-AP (same clone as 10193-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (10193-2-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10193-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RCN2 Polyclonal antibody, PBS Only

RCN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10193-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Calcium binding protein ERC 55, Calcium-binding protein ERC-55, E6 binding protein, E6-binding protein, E6BP

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10193-2-PBS targets RCN2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0247

Product name: Recombinant human RCN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-209 aa of BC004892

Sequence: KAEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLE

相同性解析による交差性が予測される生物種
Full Name reticulocalbin 2, EF-hand calcium binding domain
Calculated molecular weight 37 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC004892
Gene Symbol RCN2
Gene ID (NCBI) 5955
RRIDAB_2180018
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14257
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Reticulocalbin 2 (RCN2) is a Ca(2+)-binding protein that is specifically localized in the endoplasmic reticulum (ER). It is also termed ERC-55, i.e. ER calcium-binding protein of 55 kDa. It is a single copy gene and is encoded by an approximately 1900-base mRNA, which shows a ubiquitous expression pattern. The protein comprises an amino-terminal signal sequence followed by six copies of the EF-hand Ca2+ binding motif.

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