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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RCHY1 Monoclonal antibody

RCHY1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C11C11

Cat no : CL488-67856

Synonyms

ARNIP, CHIMP, DKFZp586C1620, hARNIP, hPirh2, p53 induced RING H2 protein, PIRH2, PRO1996, RCHY1, RING finger protein 199, RNF199, Zinc finger protein 363, ZNF363

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-67856

    IF Staining of HeLa using CL488-67856

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 RCHY1 antibody (CL488-67856, Clone: 2C11C11 ) at dilution of 1:200.

  • IF Staining of A549 using CL488-67856

    IF Staining of A549 using CL488-67856

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 RCHY1 antibody (CL488-67856, Clone: 2C11C11 ) at dilution of 1:200.

  • FC experiment of PC-12 using CL488-67856

    FC experiment of PC-12 using CL488-67856

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human RCHY1 (CL488-67856, Clone:2C11C11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inA549 cells, HeLa cells
Positive FC detected inPC-12 cells
For other applications, we recommend the unconjugated version of this antibody, 67856-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67856 targets RCHY1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RCHY1 fusion protein Ag4942 相同性解析による交差性が予測される生物種
Full Name ring finger and CHY zinc finger domain containing 1
Calculated molecular weight 261 aa, 30 kDa
GenBank accession numberBC047393
Gene symbol RCHY1
Gene ID (NCBI) 25898
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RCHY1 antibody CL488-67856Download protocol
FC protocol for CL Plus 488 RCHY1 antibody CL488-67856Download protocol
Standard Protocols
Click here to view our Standard Protocols