Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 16755-1-AP (same clone as 16755-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
WB analysis using 16755-1-AP (same clone as 16755-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 16755-1-AP (same clone as 16755-1-PBS)
PC-3 cells were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293T using 16755-1-AP (same clone as 16755-1-PBS)
HEK-293T cells were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 16755-1-AP (same clone as 16755-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 16755-1-AP (same clone as 16755-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 16755-1-AP (same clone as 16755-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 16755-1-AP (same clone as 16755-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 16755-1-AP (same clone as 16755-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 16755-1-AP (same clone as 16755-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 16755-1-AP (same clone as 16755-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 16755-1-AP (same clone as 16755-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 16755-1-AP (same clone as 16755-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
IHC staining of rat colon using 16755-1-AP (same clone as 16755-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
IHC staining of rat testis using 16755-1-AP (same clone as 16755-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.
IHC staining of rat testis using 16755-1-AP (same clone as 16755-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16755-1-PBS in a different storage buffer formulation.

RCC2 Polyclonal antibody, PBS Only

RCC2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 16755-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16755-1-PBS targets RCC2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10271 Product name: Recombinant human RCC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 168-522 aa of BC042141 Sequence: EGKLWSWGRNEKGQLGHGDTKRVEAPRLIEGLSHEVIVSAACGRNHTLALTETGSVFAFGENKMGQLGLGNQTDAVPSPAQIMYNGQPITKMACGAEFSMIMDCKGNLYSFGCPEYGQLGHNSDGKFIARAQRIEYDCELVPRRVAIFIEKTKDGQILPVPNVVVRDVACGANHTLVLDSQKRVFSWGFGGYGRLGHAEQKDEMVPRLVKLFDFPGRGASQIYAGYTCSFAVSEVGGLFFWGATNTSRESTMYPKAVQDLCGWRIRSLACGKSSIIVAADESTISWGPSPTFGELGYGDHKPKSSTAAQEVKTLDGIFSEQVAMGYSHSLVIARDESETEKEKIKKLPEYNPRTL 相同性解析による交差性が予測される生物種
Full Name	regulator of chromosome condensation 2
Calculated molecular weight	522 aa, 56 kDa
Observed molecular weight	56 kDa
GenBank accession number	BC042141
Gene Symbol	RCC2
Gene ID (NCBI)	55920
RRID	AB_2878311
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9P258
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

RCC2 (also known as TD60) is a guanine exchange factor that is active on RalA, a small GTPase. RCC2 and RalA are both essential for proper kinetochore-microtubule function in early mitosis. RCC2 has been shown to be a biomarker for colorectal cancer.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 16755-1-AP (same clone as 16755-1-PBS)

WB analysis using 16755-1-AP (same clone as 16755-1-PBS)

WB analysis of PC-3 using 16755-1-AP (same clone as 16755-1-PBS)

WB analysis of HEK-293T using 16755-1-AP (same clone as 16755-1-PBS)

IHC staining of human colon cancer using 16755-1-AP (same clone as 16755-1-PBS)

IHC staining of human colon cancer using 16755-1-AP (same clone as 16755-1-PBS)

IHC staining of human ovary tumor using 16755-1-AP (same clone as 16755-1-PBS)

IHC staining of human ovary tumor using 16755-1-AP (same clone as 16755-1-PBS)

IHC staining of human stomach cancer using 16755-1-AP (same clone as 16755-1-PBS)

IHC staining of human stomach cancer using 16755-1-AP (same clone as 16755-1-PBS)

IHC staining of mouse testis using 16755-1-AP (same clone as 16755-1-PBS)