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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RCC2 Polyclonal antibody

RCC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16755-1-AP

Synonyms

KIAA1470, Protein RCC2, RCC1 like protein TD 60, RCC2, TD 60, TD60

Validation Data Gallery

Applications:
  • WB analysis using 16755-1-AP

    WB analysis using 16755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16755-1-AP

    WB analysis of HeLa using 16755-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 16755-1-AP

    WB analysis of PC-3 using 16755-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293T using 16755-1-AP

    WB analysis of HEK-293T using 16755-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16755-1-AP

    IHC staining of human colon cancer using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16755-1-AP

    IHC staining of human colon cancer using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 16755-1-AP

    IHC staining of human ovary tumor using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 16755-1-AP

    IHC staining of human ovary tumor using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 16755-1-AP

    IHC staining of human stomach cancer using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 16755-1-AP

    IHC staining of human stomach cancer using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 16755-1-AP

    IHC staining of mouse testis using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 16755-1-AP

    IHC staining of mouse testis using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat colon using 16755-1-AP

    IHC staining of rat colon using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat colon using 16755-1-AP

    IHC staining of rat colon using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 16755-1-AP

    IHC staining of rat testis using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 16755-1-AP

    IHC staining of rat testis using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293T cells, PC-3 cells, HeLa cells, HUVEC cells, MOLT-4 cells, NCI-H1299 cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue, human stomach cancer tissue, mouse testis tissue, rat colon tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

16755-1-AP targets RCC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RCC2 fusion protein Ag10271 相同性解析による交差性が予測される生物種
Full Name regulator of chromosome condensation 2
Calculated molecular weight 522 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC042141
Gene symbol RCC2
Gene ID (NCBI) 55920
RRIDAB_2878311
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RCC2 (also known as TD60) is a guanine exchange factor that is active on RalA, a small GTPase. RCC2 and RalA are both essential for proper kinetochore-microtubule function in early mitosis. RCC2 has been shown to be a biomarker for colorectal cancer.

Protocols

Product Specific Protocols
WB protocol for RCC2 antibody 16755-1-APDownload protocol
IHC protocol for RCC2 antibody 16755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Mol Med

The m6A reader IGF2BP3 promotes acute myeloid leukemia progression by enhancing RCC2 stability.

Authors - Nan Zhang
View Article