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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RC3H1 Polyclonal antibody (31234-1-AP)

    WB analysis using 31234-1-AP (same clone as 31234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31234-1-AP (RC3H1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using RC3H1 Polyclonal antibody (31234-1-AP)

    WB analysis of HEK-293T using 31234-1-AP (same clone as 31234-1-PBS)

    WB result of RC3H1 antibody (31234-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RC3H1 transfected HEK-293T cells. This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using RC3H1 Polyclonal antibody (31234-1-AP)

    IHC staining of mouse pancreas using 31234-1-AP (same clone as 31234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RC3H1 Polyclonal antibody (31234-1-AP)

    IHC staining of human ovary cancer using 31234-1-AP (same clone as 31234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RC3H1 Polyclonal antibody (31234-1-AP)

    IHC staining of human ovary cancer using 31234-1-AP (same clone as 31234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RC3H1 Polyclonal antibody (31234-1-AP)

    IF Staining of HeLa using 31234-1-AP (same clone as 31234-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RC3H1 antibody (31234-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RC3H1 Polyclonal antibody, PBS Only

RC3H1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31234-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

KIAA2025, RING finger protein 198, RNF198, ROQUIN

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31234-1-PBS targets RC3H1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35089

Product name: Recombinant human RC3H1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1000-1133 aa of NM_001300850

Sequence: NTLAGQSQPPPPPPPKWPGMISSEQLSLELHQVEREIGKRTRELSMENQCSLDMKSKLNTSKQAENGQPEPQNKVPAEDLTLTFSDVPNGSALTQENISLLSNKTSSLNLSEDPEGGGDNNDSQRSGVTPSSAP

相同性解析による交差性が予測される生物種
Full Name ring finger and CCCH-type zinc finger domains 1
Calculated molecular weight126 kDa
Observed molecular weight130-150 kDa
GenBank accession numberNM_001300850
Gene Symbol RC3H1
Gene ID (NCBI) 149041
RRIDAB_3669912
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ5TC82
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Roquin-1 (Rc3h1) is an E3 ubiquitin ligase originally discovered in a mutational screen for genetic factors contributory to systemic lupus erythematosus-like symptoms in mice. A single base pair mutation in the Rc3h1 gene resulted in the manifestation of autoantibody production and sustained immunological inflammation characterized by excessive T follicular helper cell activation and formation of germinal centers. (PMID: 26673963)

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