CoraLite® Plus 488-conjugated RBX1 Monoclonal antibody
RBX1 Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1F4D9
Cat no : CL488-66716
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy RBX1 Ready-To-Use IHC Kit. RBX1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66716-1-Ig
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-66716 targets RBX1 in IF applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RBX1 fusion protein Ag7005 相同性解析による交差性が予測される生物種 |
| Full Name | ring-box 1 |
| Calculated molecular weight | 12 kDa |
| Observed molecular weight | 12-15 kDa |
| GenBank accession number | BC001466 |
| Gene symbol | RBX1 |
| Gene ID (NCBI) | 9978 |
| RRID | AB_2919360 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RBX1 antibody CL488-66716 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
