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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using RBP7 Polyclonal antibody (14541-1-AP)

    WB analysis of HEK-293 using 14541-1-AP (same clone as 14541-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14541-1-AP (RBP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse adipose tissue using RBP7 Polyclonal antibody (14541-1-AP)

    WB analysis of mouse adipose using 14541-1-AP (same clone as 14541-1-PBS)

    mouse adipose tissue were subjected to SDS PAGE followed by western blot with 14541-1-AP (RBP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using RBP7 Polyclonal antibody (14541-1-AP)

    IP experiment of HEK-293 using 14541-1-AP (same clone as 14541-1-PBS)

    IP result of anti-RBP7 (IP:14541-1-AP, 4ug; Detection:14541-1-AP 1:500) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RBP7 Polyclonal antibody (14541-1-AP)

    IHC staining of human colon cancer using 14541-1-AP (same clone as 14541-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14541-1-AP (RBP7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RBP7 Polyclonal antibody (14541-1-AP)

    IHC staining of human kidney using 14541-1-AP (same clone as 14541-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 14541-1-AP (RBP7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RBP7 Polyclonal antibody (14541-1-AP)

    IHC staining of human kidney using 14541-1-AP (same clone as 14541-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 14541-1-AP (RBP7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using RBP7 Polyclonal antibody (14541-1-AP)

    IF Staining of human kidney using 14541-1-AP (same clone as 14541-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using RBP7 antibody (14541-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using RBP7 Polyclonal antibody (14541-1-AP)

    IF Staining of human colon cancer using 14541-1-AP (same clone as 14541-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using RBP7 antibody (14541-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RBP7 Polyclonal antibody (14541-1-AP)

    IF Staining of HEK-293 using 14541-1-AP (same clone as 14541-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RBP7 antibody (14541-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

RBP7 Polyclonal antibody, PBS Only

RBP7 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 14541-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Synonyms

CRBP4, CRABP-IV, CRABP4, CRABP IV, Cellular retinoic acid-binding protein IV

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14541-1-PBS targets RBP7 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6045

Product name: Recombinant human RBP7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-134 aa of BC063013

Sequence: MPADLSGTWTLLSSDNFEGYMLALGIDFATRKIAKLLKPQKVIEQNGDSFTIHTNSSLRNYFVKFKVGEEFDEDNRGLDNRKCKSLVIWDNDRLTCIQKGEKKNRGWTHWIEGDKLHLEMFCEGQVCKQTFQRA

相同性解析による交差性が予測される生物種
Full Name retinol binding protein 7, cellular
Calculated molecular weight 15.5 kDa
Observed molecular weight 15-18 kDa
GenBank accession numberBC063013
Gene Symbol RBP7
Gene ID (NCBI) 116362
RRIDAB_2179293
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96R05
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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