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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBMS2 Monoclonal antibody (67395-1-Ig)

    WB analysis using 67395-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67395-1-Ig (RBMS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using RBMS2 Monoclonal antibody (67395-1-Ig)

    WB analysis of LNCaP using 67395-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67395-1-Ig (RBMS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using RBMS2 Monoclonal antibody (67395-1-Ig)

    WB analysis of U2OS using 67395-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67395-1-Ig (RBMS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RBMS2 Monoclonal antibody (67395-1-Ig)

    IHC staining of human placenta using 67395-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67395-1-Ig (RBMS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse adrenal gland tissue using RBMS2 Monoclonal antibody (67395-1-Ig)

    IHC staining of mouse adrenal gland using 67395-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67395-1-Ig (RBMS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using RBMS2 Monoclonal antibody (67395-1-Ig)

    IHC staining of mouse skeletal muscle using 67395-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67395-1-Ig (RBMS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RBMS2 Monoclonal antibody

RBMS2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67395-1-Ig
Clone No.1E5C5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

1E5C5, RNA-binding motif, single-stranded-interacting protein 2, SCR3, Suppressor of CDC2 with RNA-binding motif 3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNCI-H1299 cells, U2OS cells, LNCaP cells, A549 cells, HeLa cells
Positive IHC detected inhuman placenta tissue, mouse adrenal gland tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67395-1-Ig targets RBMS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27721

Product name: Recombinant human RBMS2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-407 aa of BC027863

Sequence: MLLSVTSRPGISTFGYNRNNKKPYVSLAQQMAPPSPSNSTPNSSSGSNGNDQLSKTNLYIRGLQPGTTDQDLVKLCQPYGKIVSTKAILDKTTNKCKGYGFVDFDSPSAAQKAVTALKASGVQAQMAKQQEQDPTNLYISNLPLSMDEQELEGMLKPFGQVISTRILRDTSGTSRGVGFARMESTEKCEAIITHFNGKYIKTPPGVPAPSDPLLCKFADGGPKKRQNQGKFVQNGRAWPRNADMGVMALTYDPTTALQNGFYPAPYNITPNRMLAQSALSPYLSSPVSSYQRVTQTSPLQVPNPSWMHHHSYLMQPSGSVLTPGMDHPISLQPASMMGPLTQQLGHLSLSSTGTYMPTAAAMQGAYISQYTPVPSSSVSVEESSGQQNQVAVDAPSEHGVYSFQFNK

相同性解析による交差性が予測される生物種
Full Name RNA binding motif, single stranded interacting protein 2
Calculated molecular weight 407 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC027863
Gene Symbol RBMS2
Gene ID (NCBI) 5939
RRIDAB_2882639
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ15434
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBMS2 (RNA-binding motif, single-stranded-interacting protein 2), also known as SCR3 (Suppressor of CDC2 with RNA-binding motif 3), is a member of c-Myc single-strand binding proteins (MSSPs) family and encodes RNA binding protein. RBMS2 plays a critical role in the regulation of transcription and alternative splicing, both key cellular processes related to cell proliferation and programmed cell death or apoptosis (PMID: 40854446). Overexpression of RBMS2 inhibited CRC cell proliferation, migration, and epithelial-mesenchymal transition (EMT) (PMID: 30514345, 38058408).

Protocols

Product Specific Protocols
IHC protocol for RBMS2 antibody 67395-1-IgDownload protocol
WB protocol for RBMS2 antibody 67395-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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