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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RBM27 Polyclonal antibody (31850-1-AP)

    WB analysis using 31850-1-AP (same clone as 31850-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31850-1-AP (RBM27 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31850-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RBM27 Polyclonal antibody (31850-1-AP)

    WB analysis of HeLa using 31850-1-AP (same clone as 31850-1-PBS)

    WB result of RBM27 antibody (31850-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM27 transfected HeLa cells. This data was developed using the same antibody clone with 31850-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RBM27 Polyclonal antibody (31850-1-AP)

    IF Staining of U2OS using 31850-1-AP (same clone as 31850-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM27 antibody (31850-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 31850-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RBM27 Polyclonal antibody, PBS Only

RBM27 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31850-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KIAA1311, RNA Binding Motif Protein 27, RNA-Binding Protein 27

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31850-1-PBS targets RBM27 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35822

Product name: Recombinant human RBM27 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 680-815 aa of NM_018989

Sequence: TLQSSAQLLLQQQQTLSHLSQQHHHLPQHLHQQQVLVAQSAPSTVHGGIQKMMSKPQTSGAYVLNKVPVKHRLGHAGGNQSDASHLLNQSGGAGEDCQIFSTPGHPKMIYSSSNLKTPSKLCSGSKSHDVQEVLKK

相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 27
Calculated molecular weight119 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_018989
Gene Symbol RBM27
Gene ID (NCBI) 54439
RRIDAB_3670129
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9P2N5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RBM27 has been identified as a candidate autism gene, and is present in cytoplasmic and and nuclear speckles. RBM27 encodes a protein containing an RNA-Binding Motif, which confers its ability to bind RNA. Therefore, RBM27 is important in mRNA processing and the turnover of nuclear polyadenylate (pA+) RNA.

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