Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, Hela cell, jurkat cell, K-562 cell |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31850-1-AP targets RBM27 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35822 Product name: Recombinant human RBM27 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 680-815 aa of NM_018989 Sequence: TLQSSAQLLLQQQQTLSHLSQQHHHLPQHLHQQQVLVAQSAPSTVHGGIQKMMSKPQTSGAYVLNKVPVKHRLGHAGGNQSDASHLLNQSGGAGEDCQIFSTPGHPKMIYSSSNLKTPSKLCSGSKSHDVQEVLKK 相同性解析による交差性が予測される生物種 |
| Full Name | RNA binding motif protein 27 |
| Calculated molecular weight | 119 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | NM_018989 |
| Gene Symbol | RBM27 |
| Gene ID (NCBI) | 54439 |
| RRID | AB_3670129 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9P2N5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBM27 has been identified as a candidate autism gene, and is present in cytoplasmic and and nuclear speckles. RBM27 encodes a protein containing an RNA-Binding Motif, which confers its ability to bind RNA. Therefore, RBM27 is important in mRNA processing and the turnover of nuclear polyadenylate (pA+) RNA.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RBM27 antibody 31850-1-AP | Download protocol |
| WB protocol for RBM27 antibody 31850-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |