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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using RBM16 Polyclonal antibody (18893-1-AP)

    WB analysis of HT-1080 using 18893-1-AP (same clone as 18893-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RBM16 Polyclonal antibody (18893-1-AP)

    WB analysis using 18893-1-AP (same clone as 18893-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RBM16 Polyclonal antibody (18893-1-AP)

    WB analysis of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using RBM16 Polyclonal antibody (18893-1-AP)

    WB analysis of NIH/3T3 using 18893-1-AP (same clone as 18893-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using RBM16 Polyclonal antibody (18893-1-AP)

    WB analysis of A549 using 18893-1-AP (same clone as 18893-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using RBM16 Polyclonal antibody (18893-1-AP)

    WB analysis of L02 using 18893-1-AP (same clone as 18893-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RBM16 Polyclonal antibody (18893-1-AP)

    IP experiment of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

    IP result of anti-RBM16 (IP:18893-1-AP, 4ug; Detection:18893-1-AP 1:800) with HeLa cells lysate 3600ug. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RBM16 Polyclonal antibody (18893-1-AP)

    IHC staining of human testis using 18893-1-AP (same clone as 18893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBM16 Polyclonal antibody (18893-1-AP)

    IHC staining of human cervical cancer using 18893-1-AP (same clone as 18893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBM16 Polyclonal antibody (18893-1-AP)

    IHC staining of human cervical cancer using 18893-1-AP (same clone as 18893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBM16 Polyclonal antibody (18893-1-AP)

    IHC staining of human cervical cancer using 18893-1-AP (same clone as 18893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBM16 Polyclonal antibody (18893-1-AP)

    IHC staining of human cervical cancer using 18893-1-AP (same clone as 18893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RBM16 Polyclonal antibody (18893-1-AP)

    IHC staining of human testis using 18893-1-AP (same clone as 18893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM16 Polyclonal antibody (18893-1-AP)

    IF Staining of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM16 antibody (18893-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBM16 Polyclonal antibody (18893-1-AP)

    IF Staining of HepG2 using 18893-1-AP (same clone as 18893-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using RBM16 antibody 18893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

RBM16 Polyclonal antibody, PBS Only

RBM16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18893-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18893-1-PBS targets RBM16 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13499

Product name: Recombinant human RBM16 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 921-1271 aa of BC070071

Sequence: MPMLDIRPGLIPQAPGPRFPLIQPGIPPQRGIPPPSVLDSALHPPPRGPFPPGDIFSQPERPFLAPGRQSVDNVTNPEKRIPLGNDNIQQEGDRDYRFPPIETRESISRPPPVDVRDVVGRPIDPREGPGRPPLDGRDHFGRPPVDIRENLVRPGIDHLGRRDHFGFNPEKPWGHRGDFDEREHRVLPVYGGPKGLHEERGRFRSGNYRFDPRSGPWNRGFGQEVHRDFDDRRRPWERQRDRDDRDFDFCREMNGNRLGRDRIQNTWVPPPHARVFDYFEGATSQRKGDNVPQVNGENTERHAQPPPIPVQNDPELYEKLTSSNEINKEKSDTVADIESEPVVESTETEGT

相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 16
Calculated molecular weight 1271 aa, 141 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC070071
Gene Symbol RBM16
Gene ID (NCBI) 22828
RRIDAB_2175908
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPN6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )

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