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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using RBBP5 Polyclonal antibody (13181-1-AP)

    WB analysis of HL-60 using 13181-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HL-60 cells using RBBP5 Polyclonal antibody (13181-1-AP)

    WB analysis of HL-60 using 13181-1-AP (same clone as 13181-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13181-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using RBBP5 Polyclonal antibody (13181-1-AP)

    WB analysis of HL-60 using 13181-1-AP (same clone as 13181-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13181-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using RBBP5 Polyclonal antibody (13181-1-AP)

    WB analysis of HL-60 using 13181-1-AP (same clone as 13181-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 13181-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RBBP5 Polyclonal antibody (13181-1-AP)

    WB analysis of HeLa using 13181-1-AP (same clone as 13181-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13181-1-PBS in a different storage buffer formulation.

RBBP5 Polyclonal antibody, PBS Only

RBBP5 Polyclonal Antibody for WB, ELISA
Cat No. 13181-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13181-1-PBS targets RBBP5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3848

Product name: Recombinant human RBBP5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-327 aa of BC037284

Sequence: MNLELLESFGQNYPEEADGTLDCISMALTCTFNRWGTLLAVGCNDGRIVIWDFLTRGIAKIISAHIHPVCSLCWSRDGHKLVSASTDNIVSQWDVLSGDCDQRFRFPSPILKVQYHPRDQNKVLVCPMKSAPVMLTLSDSKHVVLPVDDDSDLNVVASFDRRGEYIYTGNAKGKILVLKTDSQDLVASFRVTTGTSNTTAIKSIEFARKGSCFLINTADRIIRVYDGREILTCGRDGEPEPMQKLQDLVNRTPWKKCCFSGDGEYIVAGSARQHALYIWEKSIGNLVKILHGTRGELLLDVAWHPVRPIIASISSGVVSIWAQNQVE

相同性解析による交差性が予測される生物種
Full Name retinoblastoma binding protein 5
Calculated molecular weight 538 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC037284
Gene Symbol RBBP5
Gene ID (NCBI) 5929
RRIDAB_2177773
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15291
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RBQ-3, also known as RBBP5 (retinoblastoma binding protein 5) or SWD1, is a 538 amino acid protein that localizes to the nucleus and contains six WD repeats. Expressed ubiquitously, RBQ-3 functions as a component of the Set1 complex and preferentially binds to underphosphorylated forms of the retinoblastoma (Rb) protein, possibly playing a role in the regulation of cell proliferation. RBQ-3 exists as two alternatively spliced isoforms and, upon DNA damage, is subject to phosphorylation by ATM or ATR.

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