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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RBBP5 Polyclonal antibody

RBBP5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13181-1-AP

Synonyms

RBBP 5, RBBP5, RBQ3, SWD1

Validation Data Gallery

Applications:
  • WB analysis of HL-60 using 13181-1-AP

    WB analysis of HL-60 using 13181-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • WB analysis of HL-60 using 13181-1-AP

    WB analysis of HL-60 using 13181-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 13181-1-AP

    WB analysis of HL-60 using 13181-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 13181-1-AP

    WB analysis of HeLa using 13181-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 13181-1-AP

    WB analysis of HL-60 using 13181-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13181-1-AP (RBBP5 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

13181-1-AP targets RBBP5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBBP5 fusion protein Ag3848 相同性解析による交差性が予測される生物種
Full Name retinoblastoma binding protein 5
Calculated molecular weight 538 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC037284
Gene symbol RBBP5
Gene ID (NCBI) 5929
RRIDAB_2177773
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBQ-3, also known as RBBP5 (retinoblastoma binding protein 5) or SWD1, is a 538 amino acid protein that localizes to the nucleus and contains six WD repeats. Expressed ubiquitously, RBQ-3 functions as a component of the Set1 complex and preferentially binds to underphosphorylated forms of the retinoblastoma (Rb) protein, possibly playing a role in the regulation of cell proliferation. RBQ-3 exists as two alternatively spliced isoforms and, upon DNA damage, is subject to phosphorylation by ATM or ATR.

Protocols

Product Specific Protocols
WB protocol for RBBP5 antibody 13181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Authors - Gongwei Wu
View Article