Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67159 targets RASEF in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RASEF fusion protein Ag2149 相同性解析による交差性が予測される生物種 |
| Full Name | RAS and EF-hand domain containing |
| Calculated molecular weight | 740 aa, 83 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC023566 |
| Gene Symbol | RASEF |
| Gene ID (NCBI) | 158158 |
| RRID | AB_2934523 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IZ41 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The RASEF (RAS and EF-hand domain-containing protein), a member of Rab GTPase protein family, contains a Rab GTPase domain in the C-terminal region, a coiled coil motif in an internal region as well as 2 EF hand domains which were of importance for binding to calcium ions in the N-terminus.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 RASEF antibody CL488-67159 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |