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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis using 11861-1-AP (same clone as 11861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis using 11861-1-AP (same clone as 11861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of Jurkat using 11861-1-AP (same clone as 11861-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of human kidney using 11861-1-AP (same clone as 11861-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of mouse brain using 11861-1-AP (same clone as 11861-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of mouse skeletal muscle using 11861-1-AP (same clone as 11861-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of mouse heart using 11861-1-AP (same clone as 11861-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of mouse pancreas using 11861-1-AP (same clone as 11861-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of human placenta using 11861-1-AP (same clone as 11861-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of human heart using 11861-1-AP (same clone as 11861-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11861-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis using 11861-1-AP (same clone as 11861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11861-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    IHC staining of human breast cancer using 11861-1-AP (same clone as 11861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11861-1-AP (RAMP3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11861-1-PBS in a different storage buffer formulation.

RAMP3 Polyclonal antibody, PBS Only

RAMP3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11861-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11861-1-PBS targets RAMP3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2438

Product name: Recombinant human RAMP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-148 aa of BC022304

Sequence: METGALRRPQLLPLLLLLCGGCPRAGGCNETGMLERLPLCGKAFADMMGKVDVWKWCNLSEFIVYYESFTNCTEMEANVVGCYWPNPLAQGFITGIHRQFFSNCTVDRVHLEDPPDEVLIPLIVIPVVLTVAMAGLVVWRSKRTDTLL

相同性解析による交差性が予測される生物種
Full Name receptor (G protein-coupled) activity modifying protein 3
Calculated molecular weight16.5 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC022304
Gene Symbol RAMP3
Gene ID (NCBI) 10268
RRIDAB_2176465
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60896
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The receptor activity-modifying proteins (RAMPs) and the calcitonin receptor-like receptor (CRLR) are both required to generate adrenomedullin (AM) and calcitonin gene-related peptide (CGRP) receptors. During the process, RAMP3 transports the glycosylated 58-kD CGRPR to the cell surface. The formation of CRLR-RAMP heterodimer is essential for the proper cell surface targeting and pharmacological characteristics of both CGRP and AM receptors.

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