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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis using 11861-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis using 11861-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of human heart using 11861-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of human kidney using 11861-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of Jurkat using 11861-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of mouse skeletal muscle using 11861-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of mouse brain using 11861-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of mouse heart using 11861-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of mouse pancreas using 11861-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis of human placenta using 11861-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAMP3 Polyclonal antibody (11861-1-AP)

    WB analysis using 11861-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11861-1-AP (RAMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAMP3 Polyclonal antibody (11861-1-AP)

    IHC staining of human breast cancer using 11861-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11861-1-AP (RAMP3 antibody) at dilution of 1:50 (under 10x lens).

RAMP3 Polyclonal antibody

RAMP3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11861-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

RAMP3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, human kidney tissue, Jurkat cells, mouse pancreas tissue, human placenta tissue, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue, human heart tissue, mouse lung tissue, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

11861-1-AP targets RAMP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2438

Product name: Recombinant human RAMP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-148 aa of BC022304

Sequence: METGALRRPQLLPLLLLLCGGCPRAGGCNETGMLERLPLCGKAFADMMGKVDVWKWCNLSEFIVYYESFTNCTEMEANVVGCYWPNPLAQGFITGIHRQFFSNCTVDRVHLEDPPDEVLIPLIVIPVVLTVAMAGLVVWRSKRTDTLL

相同性解析による交差性が予測される生物種
Full Name receptor (G protein-coupled) activity modifying protein 3
Calculated molecular weight16.5 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC022304
Gene Symbol RAMP3
Gene ID (NCBI) 10268
RRIDAB_2176465
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60896
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The receptor activity-modifying proteins (RAMPs) and the calcitonin receptor-like receptor (CRLR) are both required to generate adrenomedullin (AM) and calcitonin gene-related peptide (CGRP) receptors. During the process, RAMP3 transports the glycosylated 58-kD CGRPR to the cell surface. The formation of CRLR-RAMP heterodimer is essential for the proper cell surface targeting and pharmacological characteristics of both CGRP and AM receptors.

Protocols

Product Specific Protocols
IHC protocol for RAMP3 antibody 11861-1-APDownload protocol
WB protocol for RAMP3 antibody 11861-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Chin Med Assoc

WGCNA reveals a biomarker for cancer-associated fibroblasts to predict prognosis in cervical cancer

Authors - Zao-Ling Liu
View Article
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