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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite®594-conjugated RALY Monoclonal antibody (CL594-68011)

    IF Staining of A431 using CL594-68011

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 RALY antibody (CL594-68011, Clone: 2B10C3 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite®594-conjugated RALY Monoclonal antibody (CL594-68011)

    FC experiment of A431 using CL594-68011

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RALY (CL594-68011, Clone:2B10C3) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated RALY Monoclonal antibody

RALY Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-68011
Clone No.2B10C3

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Synonyms

HNRPCL2, hnRNP core protein C-like 2, hnRNP associated with lethal yellow protein homolog, Heterogeneous nuclear ribonucleoprotein C-like 2, Autoantigen p542

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-68011 targets RALY in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12250

Product name: Recombinant human RALY protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-307 aa of BC103753

Sequence: MSLKLQASNVTNKNDPKSINSRVFIGNLNTALVKKSDVETIFSKYGRVAGCSVHKGYAFVQYSNERHARAAVLGENGRVLAGQTLDINMAGEPKPDRPKGLKRAASAIYSGYIFDYDYYRDDFYDRLFDYRGRLSPVPVPRAVPVKRPRVTVPLVRRVKTNVPVKLFARSTAVTTSSAKIKLKSSELQAIKTELTQIKSNIDALLSRLEQIAAEQKANPDGKKKGDGGGASGGGGGGGGSGGGGSGGGGGGGSSRPPAPQENTTSEAGLPQGEARTRDDGDEEGLLTHSEEELEHSQDTDADDGALQ

相同性解析による交差性が予測される生物種
Full Name RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog (mouse))
Calculated molecular weight 307 aa, 33 kDa
Observed molecular weight37 kDa
GenBank accession numberBC103753
Gene Symbol RALY
Gene ID (NCBI) 22913
RRIDAB_3084792
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9UKM9
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA-binding protein RALY is a protein that in humans is encoded by the RALY gene. In infectious mononucleosis, anti-EBNA-1 antibodies are produced which cross-react with multiple normal human proteins. The cross-reactivity is due to anti-gly/ala antibodies that cross-react with host proteins containing configurations like those in the EBNA-1 repeat. One such antigen is RALY which is a member of the heterogeneous nuclear ribonucleoprotein gene family.

Protocols

Product Specific Protocols
IF protocol for CL594 RALY antibody CL594-68011Download protocol
FC protocol for CL594 RALY antibody CL594-68011Download protocol
Standard Protocols
Click here to view our Standard Protocols