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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAGE Recombinant antibody (83742-5-RR)

    WB analysis using 83742-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83742-5-RR (RAGE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Ager/Rage Recombinant antibody (83742-5-RR)

    IHC staining of mouse lung using 83742-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83742-5-RR (Ager/Rage antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using Ager/Rage Recombinant antibody (83742-5-RR)

    IF Staining of mouse lung using 83742-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Ager/Rage antibody (83742-5-RR, Clone: 240774F11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83742-5-RR

    Affinity and Kinetic Characterization of 83742-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Human ATM were performed. The affinity constant is 2.58 nM. Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Mouse RAGE were performed. The affinity constant is 6.41 nM.

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RAGE Recombinant antibody

RAGE Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83742-5-RR
Clone No.240774F11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Ager, RAGE1, RAGE 1, MOK, 240774F11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat lung tissue, mouse lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83742-5-RR targets RAGE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name advanced glycosylation end product-specific receptor
Calculated molecular weight43 kDa
Observed molecular weight42-55 kDa
GenBank accession numberNM_007425.3
Gene Symbol Ager
Gene ID (NCBI) 11596
RRIDAB_3671340
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ62151-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The receptor for advanced glycation end products (RAGE) is a cell surface transmembrane multiligand receptor, also known as Ager, is a multiligand member of the immunoglobulin superfamily. It has many isoforms and is predominantly expressed in the lung. AGER expression is upregulated in many diseases such as Alzheimer's disease, diabetes mellitus, atherosclerosis, and rheumatoid arthritis (PMID: 35099614).

Protocols

Product Specific Protocols
WB protocol for RAGE antibody 83742-5-RRDownload protocol
IHC protocol for RAGE antibody 83742-5-RRDownload protocol
IF protocol for RAGE antibody 83742-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols