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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAGE Recombinant antibody (83742-5-RR)

    WB analysis using 83742-5-RR (same clone as 83742-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83742-5-RR (RAGE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83742-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using RAGE Recombinant antibody (83742-5-RR)

    IHC staining of mouse lung using 83742-5-RR (same clone as 83742-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83742-5-RR (Ager/Rage antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83742-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using RAGE Recombinant antibody (83742-5-RR)

    IF Staining of mouse lung using 83742-5-RR (same clone as 83742-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Ager/Rage antibody (83742-5-RR, Clone: 240774F11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83742-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83742-5-RR

    Affinity and Kinetic Characterization of 83742-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Human ATM were performed. The affinity constant is 2.58 nM. Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Mouse RAGE were performed. The affinity constant is 6.41 nM.

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Mouse RAGE Recombinant antibody, PBS Only

RAGE Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83742-5-PBS
Clone No.240774F11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Ager, RAGE1, RAGE 1, MOK, 240774F11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83742-5-PBS targets RAGE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name advanced glycosylation end product-specific receptor
Calculated molecular weight43 kDa
Observed molecular weight42-55 kDa
GenBank accession numberNM_007425.3
Gene Symbol Ager
Gene ID (NCBI) 11596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ62151-1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The receptor for advanced glycation end products (RAGE) is a cell surface transmembrane multiligand receptor, also known as Ager, is a multiligand member of the immunoglobulin superfamily. It has many isoforms and is predominantly expressed in the lung. AGER expression is upregulated in many diseases such as Alzheimer's disease, diabetes mellitus, atherosclerosis, and rheumatoid arthritis (PMID: 35099614).