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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAC1 Monoclonal antibody (66122-1-Ig)

    WB analysis using 66122-1-Ig (same clone as 66122-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66122-1-Ig (Rac1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using RAC1 Monoclonal antibody (66122-1-Ig)

    WB analysis of NIH/3T3 using 66122-1-Ig (same clone as 66122-1-PBS)

    WB result of RAC1 antibody (66122-1-Ig, 1:2000) with si-control and si-RAC1 transfected NIH3T3 cells. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RAC1 Monoclonal antibody (66122-1-Ig)

    WB analysis of HeLa using 66122-1-Ig (same clone as 66122-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66122-1-Ig (Rac1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using RAC1 Monoclonal antibody (66122-1-Ig)

    WB analysis of human brain using 66122-1-Ig (same clone as 66122-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66122-1-Ig (RAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HL-60 cells using RAC1 Monoclonal antibody (66122-1-Ig)

    IP experiment of HL-60 using 66122-1-Ig (same clone as 66122-1-PBS)

    IP result of anti-RAC1 (IP:66122-1-Ig, 3ug; Detection:66122-1-Ig 1:700) with HL-60 cells lysate 4000ug. This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAC1 Monoclonal antibody (66122-1-Ig)

    IHC staining of human colon cancer using 66122-1-Ig (same clone as 66122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66122-1-Ig (RAC1 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAC1 Monoclonal antibody (66122-1-Ig)

    IHC staining of human colon cancer using 66122-1-Ig (same clone as 66122-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66122-1-Ig (RAC1 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RAC1 Monoclonal antibody (66122-1-Ig)

    IF Staining of NIH/3T3 using 66122-1-Ig (same clone as 66122-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed NIH/3T3 cells using 66122-1-Ig(Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAC1 Monoclonal antibody (66122-1-Ig)

    IF Staining of HeLa using 66122-1-Ig (same clone as 66122-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66122-1-Ig(Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66122-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RAC1 Monoclonal antibody, PBS Only

RAC1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 66122-1-PBS
Clone No.4A4B11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66122-1-PBS targets RAC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag17779

Product name: Recombinant human RAC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-192 aa of BC004247

Sequence: MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLIPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL

相同性解析による交差性が予測される生物種
Full Name ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC004247
Gene Symbol RAC1
Gene ID (NCBI) 5879
RRIDAB_2881521
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP63000
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

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