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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RABIF Polyclonal antibody (29113-1-AP)

    WB analysis using 29113-1-AP (same clone as 29113-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29113-1-AP (RABIF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RABIF Polyclonal antibody (29113-1-AP)

    IHC staining of mouse brain using 29113-1-AP (same clone as 29113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29113-1-AP (RABIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RABIF Polyclonal antibody (29113-1-AP)

    IHC staining of human breast cancer using 29113-1-AP (same clone as 29113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29113-1-AP (RABIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RABIF Polyclonal antibody (29113-1-AP)

    IF Staining of MCF-7 using 29113-1-AP (same clone as 29113-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RABIF antibody (29113-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29113-1-PBS in a different storage buffer formulation.

RABIF Polyclonal antibody, PBS Only

RABIF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29113-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29113-1-PBS targets RABIF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30761

Product name: Recombinant human RABIF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-72 aa of BC018488

Sequence: MEPAEQPSELVSAEGRNRKAVLCQRCGSRVLQPGTALFSRRQLFLPSMRKKPALSDGSNPDGDLLQEHWLVE

相同性解析による交差性が予測される生物種
Full Name RAB interacting factor
Calculated molecular weight 123 aa, 14 kDa
Observed molecular weight14 kDa
GenBank accession numberBC018488
Gene Symbol RABIF
Gene ID (NCBI) 5877
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP47224
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RABIF (Rab-interacting factor)/MSS4 (mammalian suppressor of Sec4) was the first putative guanine nucleotide exchange factors (GEFs) isolated for Rabs, which may act as a chaperone, allowing interacting Rab proteins to be properly activated where and when they are needed in the cell (PMID: 25158853). RABIF is a 17-kDa protein that is expressed in all mammalian tissues and is in complex with nucleotide-free Rab8 GTPase and Rab10 GTPase (PMID: 28894007, 28228250).

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