Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | mouse brain tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29113-1-AP targets RABIF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30761 Product name: Recombinant human RABIF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-72 aa of BC018488 Sequence: MEPAEQPSELVSAEGRNRKAVLCQRCGSRVLQPGTALFSRRQLFLPSMRKKPALSDGSNPDGDLLQEHWLVE 相同性解析による交差性が予測される生物種 |
| Full Name | RAB interacting factor |
| Calculated molecular weight | 123 aa, 14 kDa |
| Observed molecular weight | 14 kDa |
| GenBank accession number | BC018488 |
| Gene Symbol | RABIF |
| Gene ID (NCBI) | 5877 |
| RRID | AB_3669674 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P47224 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RABIF (Rab-interacting factor)/MSS4 (mammalian suppressor of Sec4) was the first putative guanine nucleotide exchange factors (GEFs) isolated for Rabs, which may act as a chaperone, allowing interacting Rab proteins to be properly activated where and when they are needed in the cell (PMID: 25158853). RABIF is a 17-kDa protein that is expressed in all mammalian tissues and is in complex with nucleotide-free Rab8 GTPase and Rab10 GTPase (PMID: 28894007, 28228250).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RABIF antibody 29113-1-AP | Download protocol |
| IHC protocol for RABIF antibody 29113-1-AP | Download protocol |
| WB protocol for RABIF antibody 29113-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |