• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RABEPK/p40 Monoclonal antibody

RABEPK/p40 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E11A3

Cat no : CL488-66622

Synonyms

40 kDa Rab9 effector protein, bA65N13.1, DKFZp686P1077, p40, RAB9P40, RABEPK

Validation Data Gallery

Applications:
  • IF Staining of A549 using CL488-66622

    IF Staining of A549 using CL488-66622

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 RABEPK/p40 antibody (CL488-66622, Clone: 1E11A3 ) at dilution of 1:200.

Tested Applications

Positive IF detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 66622-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66622 targets RABEPK/p40 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RABEPK/p40 fusion protein Ag7796 相同性解析による交差性が予測される生物種
Full Name Rab9 effector protein with kelch motifs
Calculated molecular weight 41 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC065725
Gene symbol RABEPK
Gene ID (NCBI) 10244
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RABEPK/p40 antibody CL488-66622Download protocol
Standard Protocols
Click here to view our Standard Protocols