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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated RAB5A Monoclonal antibody

RAB5A Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B6A5

Cat no : CL488-66339

Synonyms

RAB5, RAB5A, Ras related protein Rab 5A

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-66339

    IF Staining of HeLa using CL488-66339

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RAB5A antibody (CL488-66339, Clone: 1B6A5 ) at dilution of 1:200, CL594-Phalloidin (red).

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66339-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66339 targets RAB5A in IF applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RAB5A fusion protein Ag16781 相同性解析による交差性が予測される生物種
Full Name RAB5A, member RAS oncogene family
Calculated molecular weight 215 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC001267
Gene symbol RAB5A
Gene ID (NCBI) 5868
RRIDAB_2883313
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion. This anitbody is CL488 conjugated.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 RAB5A antibody CL488-66339Download protocol
Standard Protocols
Click here to view our Standard Protocols