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Validation Data Gallery

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  • Western Blot (WB) analysis of NCI-H1299 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    WB analysis of NCI-H1299 using 67118-1-Ig (same clone as 67118-1-PBS)

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67118-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    WB analysis of A549 using 67118-1-Ig (same clone as 67118-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67118-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    WB analysis of HT-29 using 67118-1-Ig (same clone as 67118-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67118-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    WB analysis of THP-1 using 67118-1-Ig (same clone as 67118-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB3D Monoclonal antibody (67118-1-Ig)

    IHC staining of human colon cancer using 67118-1-Ig (same clone as 67118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67118-1-Ig (RAB3D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67118-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB3D Monoclonal antibody (67118-1-Ig)

    IHC staining of human colon cancer using 67118-1-Ig (same clone as 67118-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67118-1-Ig (RAB3D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67118-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    IF Staining of A549 using 67118-1-Ig (same clone as 67118-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RAB3D antibody (67118-1-Ig, Clone: 3C2F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67118-1-PBS in a different storage buffer formulation.

RAB3D Monoclonal antibody, PBS Only

RAB3D Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67118-1-PBS
Clone No.3C2F10

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

D2 2, GOV, RAB16, RAB3D, RAD3D, Ras related protein Rab 3D

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67118-1-PBS targets RAB3D in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag16784

Product name: Recombinant human RAB3D protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-219 aa of BC016471

Sequence: MASAGDTQAGPRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDIANQESFAAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVPAEDGRRLADDLGFEFFEASAKENINVKQVFERLVDVICEKMNESLEPSSSSGSNGKGPAVGDAPAPQPSSCSC

相同性解析による交差性が予測される生物種
Full Name RAB3D, member RAS oncogene family
Calculated molecular weight 219 aa, 24 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC016471
Gene Symbol RAB3D
Gene ID (NCBI) 9545
RRIDAB_2882421
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95716
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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