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Validation Data Gallery

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  • Western Blot (WB) analysis of NCI-H1299 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    WB analysis of NCI-H1299 using 67118-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    WB analysis of A549 using 67118-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    WB analysis of HT-29 using 67118-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of THP-1 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    WB analysis of THP-1 using 67118-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67118-1-Ig (RAB3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB3D Monoclonal antibody (67118-1-Ig)

    IHC staining of human colon cancer using 67118-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67118-1-Ig (RAB3D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RAB3D Monoclonal antibody (67118-1-Ig)

    IHC staining of human colon cancer using 67118-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67118-1-Ig (RAB3D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using RAB3D Monoclonal antibody (67118-1-Ig)

    IF Staining of A549 using 67118-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RAB3D antibody (67118-1-Ig, Clone: 3C2F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

RAB3D Monoclonal antibody

RAB3D Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67118-1-Ig
Clone No.3C2F10

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3C2F10, GOV, RAB16, RAD3D, Ras related protein Rab 3D

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNCI-H1299 cells, A549 cells, HT-29 cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67118-1-Ig targets RAB3D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag16784

Product name: Recombinant human RAB3D protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-219 aa of BC016471

Sequence: MASAGDTQAGPRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDIANQESFAAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVPAEDGRRLADDLGFEFFEASAKENINVKQVFERLVDVICEKMNESLEPSSSSGSNGKGPAVGDAPAPQPSSCSC

相同性解析による交差性が予測される生物種
Full Name RAB3D, member RAS oncogene family
Calculated molecular weight 219 aa, 24 kDa
Observed molecular weight 24-27 kDa
GenBank accession numberBC016471
Gene Symbol RAB3D
Gene ID (NCBI) 9545
RRIDAB_2882421
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95716
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for RAB3D antibody 67118-1-IgDownload protocol
IHC protocol for RAB3D antibody 67118-1-IgDownload protocol
WB protocol for RAB3D antibody 67118-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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