Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 67837-1-Ig (same clone as 67837-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67837-1-Ig (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 67837-1-Ig (same clone as 67837-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67837-1-Ig (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 67837-1-Ig (same clone as 67837-1-PBS)
WB result of RAB1B antibody (67837-1-Ig; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB1B transfected HeLa cells. This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67837-1-Ig (same clone as 67837-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67837-1-Ig (same clone as 67837-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 67837-1-Ig (same clone as 67837-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 67837-1-Ig (same clone as 67837-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IHC staining of rat heart using 67837-1-Ig (same clone as 67837-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IHC staining of rat heart using 67837-1-Ig (same clone as 67837-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67837-1-Ig (same clone as 67837-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 67837-1-Ig (same clone as 67837-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 67837-1-Ig (same clone as 67837-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 67837-1-Ig (same clone as 67837-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 67837-1-Ig (same clone as 67837-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 67837-1-Ig (same clone as 67837-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IF Staining of MCF-7 using 67837-1-Ig (same clone as 67837-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RAB1B antibody (67837-1-Ig, Clone: 3C2G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.
IF Staining of NIH/3T3 using 67837-1-Ig (same clone as 67837-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAB1B antibody (67837-1-Ig, Clone: 3C2G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67837-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

RAB1B Monoclonal antibody, PBS Only

RAB1B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67837-1-PBS

Clone No.3C2G6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3C2G6, EC:3.6.5.2, Ras related protein Rab 1B, Ras-related protein Rab-1B

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67837-1-PBS targets RAB1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag12069 Product name: Recombinant human RAB1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-201 aa of BC071169 Sequence: MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC 相同性解析による交差性が予測される生物種
Full Name	RAB1B, member RAS oncogene family
Calculated molecular weight	201 aa, 22 kDa
Observed molecular weight	22 kDa
GenBank accession number	BC071169
Gene Symbol	RAB1B
Gene ID (NCBI)	81876
RRID	AB_2918599
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9H0U4
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 67837-1-Ig (same clone as 67837-1-PBS)

WB analysis of NIH/3T3 using 67837-1-Ig (same clone as 67837-1-PBS)

WB analysis of HeLa using 67837-1-Ig (same clone as 67837-1-PBS)

IHC staining of human lung cancer using 67837-1-Ig (same clone as 67837-1-PBS)

IHC staining of human lung cancer using 67837-1-Ig (same clone as 67837-1-PBS)

IHC staining of human pancreas cancer using 67837-1-Ig (same clone as 67837-1-PBS)

IHC staining of human pancreas cancer using 67837-1-Ig (same clone as 67837-1-PBS)

IHC staining of rat heart using 67837-1-Ig (same clone as 67837-1-PBS)

IHC staining of rat heart using 67837-1-Ig (same clone as 67837-1-PBS)

IHC staining of human liver cancer using 67837-1-Ig (same clone as 67837-1-PBS)

IHC staining of human stomach cancer using 67837-1-Ig (same clone as 67837-1-PBS)

IHC staining of human stomach cancer using 67837-1-Ig (same clone as 67837-1-PBS)

IHC staining of rat brain using 67837-1-Ig (same clone as 67837-1-PBS)