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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB1B Monoclonal antibody (67837-1-Ig)

    WB analysis using 67837-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67837-1-Ig (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RAB1B Monoclonal antibody (67837-1-Ig)

    WB analysis of NIH/3T3 using 67837-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67837-1-Ig (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RAB1B Monoclonal antibody (67837-1-Ig)

    WB analysis of HeLa using 67837-1-Ig

    WB result of RAB1B antibody (67837-1-Ig; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB1B transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RAB1B Monoclonal antibody (67837-1-Ig)

    IHC staining of human lung cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RAB1B Monoclonal antibody (67837-1-Ig)

    IHC staining of human lung cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RAB1B Monoclonal antibody (67837-1-Ig)

    IHC staining of human pancreas cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RAB1B Monoclonal antibody (67837-1-Ig)

    IHC staining of human pancreas cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using RAB1B Monoclonal antibody (67837-1-Ig)

    IHC staining of rat heart using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using RAB1B Monoclonal antibody (67837-1-Ig)

    IHC staining of rat heart using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RAB1B Monoclonal antibody (67837-1-Ig)

    IHC staining of human liver cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RAB1B Monoclonal antibody (67837-1-Ig)

    IHC staining of human stomach cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RAB1B Monoclonal antibody (67837-1-Ig)

    IHC staining of human stomach cancer using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using RAB1B Monoclonal antibody (67837-1-Ig)

    IHC staining of rat brain using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using RAB1B Monoclonal antibody (67837-1-Ig)

    IHC staining of rat brain using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RAB1B Monoclonal antibody (67837-1-Ig)

    IHC staining of human ovary tumor using 67837-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RAB1B Monoclonal antibody (67837-1-Ig)

    IF Staining of MCF-7 using 67837-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RAB1B antibody (67837-1-Ig, Clone: 3C2G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using RAB1B Monoclonal antibody (67837-1-Ig)

    IF Staining of NIH/3T3 using 67837-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAB1B antibody (67837-1-Ig, Clone: 3C2G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

RAB1B Monoclonal antibody

RAB1B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67837-1-Ig
Clone No.3C2G6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3C2G6, EC:3.6.5.2, Ras related protein Rab 1B, Ras-related protein Rab-1B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, NIH/3T3 cells, HeLa cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, human stomach cancer tissue, rat brain tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67837-1-Ig targets RAB1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12069

Product name: Recombinant human RAB1B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-201 aa of BC071169

Sequence: MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC

相同性解析による交差性が予測される生物種
Full Name RAB1B, member RAS oncogene family
Calculated molecular weight 201 aa, 22 kDa
Observed molecular weight22 kDa
GenBank accession numberBC071169
Gene Symbol RAB1B
Gene ID (NCBI) 81876
RRIDAB_2918599
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9H0U4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for RAB1B antibody 67837-1-IgDownload protocol
IHC protocol for RAB1B antibody 67837-1-IgDownload protocol
WB protocol for RAB1B antibody 67837-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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