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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Piccolo Polyclonal antibody (18156-1-AP)

    IF Staining of mouse brain using 18156-1-AP (same clone as 18156-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Piccolo antibody (18156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18156-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Piccolo Polyclonal antibody (18156-1-AP)

    IF Staining of mouse brain using 18156-1-AP (same clone as 18156-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Piccolo antibody (18156-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18156-1-PBS in a different storage buffer formulation.

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Piccolo Polyclonal antibody, PBS Only

Piccolo Polyclonal Antibody for IF-P, Indirect ELISA
Cat No. 18156-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18156-1-PBS targets Piccolo in IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Piccolo fusion protein Ag12952 相同性解析による交差性が予測される生物種
Full Name piccolo (presynaptic cytomatrix protein)
Calculated molecular weight 553 kDa
GenBank accession numberBC001304
Gene Symbol Piccolo
Gene ID (NCBI) 27445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6V0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Piccolo is a presynaptic scaffolding protein of the cytomatrix assembled at the active zone (AZ). AZ is a special region of the presynaptic plasma membrane where synaptic vesicles (SVs) fuse. It has been shown that Piccolo affects presynaptic function by negatively regulating SV exocytosis (PMID: 18519737).