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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Piccolo Polyclonal antibody (18156-1-AP)

    IF Staining of mouse brain using 18156-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Piccolo antibody (18156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Piccolo Polyclonal antibody (18156-1-AP)

    IF Staining of mouse brain using 18156-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Piccolo antibody (18156-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Piccolo Polyclonal antibody

Piccolo Polyclonal Antibody for IF-P, ELISA
Cat No. 18156-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, ELISA

Synonyms

ACZ, Aczonin, KIAA0559, PCLO, Piccolo, Protein piccolo

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18156-1-AP targets Piccolo in IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12952

Product name: Recombinant human PCLO protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-356 aa of BC001304

Sequence: MKKFRVSLVSKVGKQKYVDLNMLSDSENSQHLELHEPPKAVDKAKSPGVDPKQLAAELQKVSLQQSPLVLSSVVEKGSHVHSGPTSAGSSSVPSPGQPGSPSVSKKKHGSSKPTDGTKVVSHPITGEIQLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGAEYKRRTKHVQKSLNPEWNQTVIYKSISMEQLKKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTAHLDNTPRWYPLKEQTESIDHGKSHSSQSSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVTQTHLEDAGAAIAAAEAAVQQLRIQPSKRRK

相同性解析による交差性が予測される生物種
Full Name piccolo (presynaptic cytomatrix protein)
Calculated molecular weight 553 kDa
GenBank accession numberBC001304
Gene Symbol Piccolo
Gene ID (NCBI) 27445
RRIDAB_3085556
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6V0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Piccolo is a presynaptic scaffolding protein of the cytomatrix assembled at the active zone (AZ). AZ is a special region of the presynaptic plasma membrane where synaptic vesicles (SVs) fuse. It has been shown that Piccolo affects presynaptic function by negatively regulating SV exocytosis (PMID: 18519737).

Protocols

Product Specific Protocols
IF protocol for Piccolo antibody 18156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols