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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Phospho-mTOR (Ser2448) Recombinant antibody, PBS Only

Phospho-mTOR (Ser2448) Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3L18

Cat no : 80596-1-PBS

Synonyms

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2448), RAFT1, Rapamycin target protein 1, RAPT1

Validation Data Gallery

Applications:
  • WB analysis using 80596-1-RR (same clone as 80596-1-PBS)

    WB analysis using 80596-1-RR (same clone as 80596-1-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80596-1-RR (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) subsequently. This data was developed using the same antibody clone with 80596-1-PBS in a different storage buffer formulation.

  • WB analysis using 80596-1-RR (same clone as 80596-1-PBS)

    WB analysis using 80596-1-RR (same clone as 80596-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80596-1-RR (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) subsequently. This data was developed using the same antibody clone with 80596-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 80596-1-RR (same clone as 80596-1-PBS)

    WB analysis of HSC-T6 using 80596-1-RR (same clone as 80596-1-PBS)

    Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80596-1-RR (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80596-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 80596-1-RR (same clone as 80596-1-PBS)

    IF Staining of HEK-293 using 80596-1-RR (same clone as 80596-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PMA treated HEK-293 cells using Phospho-mTOR (Ser2448) antibody (80596-1-RR, Clone: 3L18 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 80596-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

80596-1-PBS targets Phospho-mTOR (Ser2448) in WB, IF, Indirect ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 12-rapamycin associated protein 1
Calculated molecular weight 289 kDa
Observed molecular weight250-289 kDa
GenBank accession numberBC117166
Gene symbol FRAP1
Gene ID (NCBI) 2475
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.