Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, λ phosphatase treated HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30811-1-AP targets Phospho-eEF2 (Thr57) in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic elongation factor-2 kinase |
| Calculated molecular weight | 725 aa, 100 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC032665 |
| Gene Symbol | EEF2K |
| Gene ID (NCBI) | 29904 |
| RRID | AB_3669761 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00418 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
eEF2 (Eukaryotic Elongation Factor 2) is a key component in the process of protein synthesis in eukaryotic cells. It is a GTP-binding protein that plays a crucial role in the elongation phase of translation. Specifically, eEF2 facilitates the movement of the ribosome along the mRNA, allowing the addition of amino acids to the growing polypeptide chain. This elongation factor is essential for efficient and accurate protein synthesis. Interestingly, eEF2 phosphorylation can either be increased or decreased depending on the stimulus used in learning or synaptic plasticity paradigms. Examining the specific contributions of increases and decreases in eEF2 phosphorylation to learning and memory is difficult. (PMID: 25762683)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-eEF2 (Thr57) antibody 30811-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |