Validation Data Gallery
Tested Applications
| Positive WB detected in | Calyculin A treated HeLa cells, PANC-1 cells, Calyculin A treated NIH/3T3 cells, Calyculin A treated HSC-T6 cells |
| Positive FC (Intra) detected in | Calyculin A treated HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87714-1-RR targets Phospho-Vimentin (Ser39) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | vimentin |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | NM_003380 |
| Gene Symbol | VIM |
| Gene ID (NCBI) | 7431 |
| ENSEMBL Gene ID | ENSG00000026025 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08670 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vimentin, a kind of intermediate filament protein III in mesenchymal cells, has become a highly researched topic around the world in recent years, as it holds complex biological functions and plays an important role in the epithelial-mesenchymal transition in the evolution of various tumors. Mesenchymal stem cells (MSCs) are widely studied for their application in cell therapy. The binding of AKT (tail region) to Vim (head region) results in Vim Ser39 phosphorylation enhancing the ability of Vim to induce motility and invasion while protecting Vim from caspase-induced proteolysis. (PMID: 33378968, PMID: 20856200, PMID: 31972217)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Phospho-Vimentin (Ser39) antibody 87714-1-RR | Download protocol |
| WB protocol for Phospho-Vimentin (Ser39) antibody 87714-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |