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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-TAU (Thr217) Polyclonal antibody (31138-1-AP)

    WB analysis using 31138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31138-1-AP (Phospho-TAU (Thr217) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. The FAD3T mouse is a triple transgenic model expressing APP, PS1, and Tau transgenes.

  • Western Blot (WB) analysis of various lysates using Phospho-TAU (Thr217) Polyclonal antibody (31138-1-AP)

    WB analysis using 31138-1-AP

    Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 31138-1-AP (Phospho-TAU (Thr217) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-TAU (Thr217) Polyclonal antibody

Phospho-TAU (Thr217) Polyclonal Antibody for WB, ELISA
Cat No. 31138-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

p Tau, p Tau (Thr217), p Tau Thr217, Phospho Tau, Phospho Tau (Thr217)

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inFAD3T mouse brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31138-1-AP targets Phospho-TAU (Thr217) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein tau
Calculated molecular weight 37-46, 79-81 kDa
Observed molecular weight70 kDa
GenBank accession numberBC000558
Gene Symbol TAU
Gene ID (NCBI) 4137
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP10636
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Phosphorylated tau at threonine 217 (p-tau217) is one of the emerging tau markers that has repeatedly shown more promise than other p-tau isoforms as a marker of AD pathology.

Protocols

Product Specific Protocols
WB protocol for Phospho-TAU (Thr217) antibody 31138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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