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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using Phospho-TAU(Ser409) Recombinant monoclonal antibod (87019-1-RR)

    WB analysis of mouse brain using 87019-1-RR

    Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 87019-1-RR (Phospho-TAU(Ser409) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Phospho-TAU (Ser409) Recombinant monoclonal antibo (87019-1-RR)

    IF Staining of mouse brain using 87019-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded λ phosphatase treated mouse brain tissue using Phospho-TAU (Ser409) antibody (87019-1-RR, Clone: 252113B1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Phospho-TAU (Ser409) Recombinant monoclonal antibody

Phospho-TAU (Ser409) Uni-rAb® Recombinant Antibody for WB, IF-P, ELISA
Cat No. 87019-1-RR
Clone No.252113B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

p TAU, p TAU (Ser409), p TAU Ser409, phos TAU, Phospho TAU

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87019-1-RR targets Phospho-TAU (Ser409) in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name microtubule-associated protein tau
Calculated molecular weight 37-46, 79-81 kDa
Observed molecular weight45-60 kDa
GenBank accession numberBC000558
Gene Symbol TAU
Gene ID (NCBI) 4137
RRIDAB_3745278
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP10636
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phosphorylation of tau at Ser409 is an early, disease-specific event in Alzheimer's brain. Catalyzed by PKA, this modification weakens tau-microtubule binding and acts as a molecular switch that initiates tau self-aggregation into paired helical filaments. Consequently, p-Ser409-tau is recognized as an early diagnostic epitope and a potential target for interrupting PKA-driven, oxidative-stress-linked tau pathology.

Protocols

Product Specific Protocols
IF protocol for Phospho-TAU (Ser409) antibody 87019-1-RRDownload protocol
WB protocol for Phospho-TAU (Ser409) antibody 87019-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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