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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-SHP2 (Tyr580) Recombinant monoclonal antib (81219-2-RR)

    WB analysis using 81219-2-RR

    Non-treated C6 cells and PDGF (HZ-1215) treated C6 cells were subjected to SDS PAGE followed by western blot with 81219-2-RR (Phospho-SHP2 (Tyr580) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-SHP2 (Tyr580) Recombinant monoclonal antib (81219-2-RR)

    WB analysis using 81219-2-RR

    Non-treated NIH/3T3 cells and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81219-2-RR (Phospho-SHP2 (Tyr580) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

Phospho-SHP2 (Tyr580) Recombinant monoclonal antibody

Phospho-SHP2 (Tyr580) Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 81219-2-RR
Clone No.242434E2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

P SHP2 (Tyr580), Phospho SHP-2 (Tyr580), Phospho-SHP2, Phospho-SHP-2, Phospho-SHP-2 (Tyr580)

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPDGF treated NIH/3T3 cells, PDGF treated C6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81219-2-RR targets Phospho-SHP2 (Tyr580) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated molecular weight 597 aa, 68 kDa
Observed molecular weight72 kDa
GenBank accession numberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ06124
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Shp2, encoded by the PTPN11 gene, is a protein tyrosine phosphatase that acts as a positive regulator of Ras-Erk pathway. Shp2 contains two tandem Src homology 2 (SH2) domains, a protein tyrosine phosphatase domain and tyrosine phosphorylation sites. It has been suggested that the phosphorylated Tyr542 and Tyr580 recruit Grb2/Sos complex to activate Ras-Erk signaling. Recently, it was reported that ZAP70, a non-receptor tyrosine kinase, enhances phosphorylation of Shp2 at Tyr580 and activates Erk. (PMID: 23318428)

Protocols

Product Specific Protocols
WB protocol for Phospho-SHP2 (Tyr580) antibody 81219-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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