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Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
81219-2-PBS targets Phospho-SHP2 (Tyr580) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | protein tyrosine phosphatase, non-receptor type 11 |
| Calculated molecular weight | 597 aa, 68 kDa |
| Observed molecular weight | 72 kDa |
| GenBank accession number | BC008692 |
| Gene Symbol | PTPN11 |
| Gene ID (NCBI) | 5781 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q06124 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
Shp2, encoded by the PTPN11 gene, is a protein tyrosine phosphatase that acts as a positive regulator of Ras-Erk pathway. Shp2 contains two tandem Src homology 2 (SH2) domains, a protein tyrosine phosphatase domain and tyrosine phosphorylation sites. It has been suggested that the phosphorylated Tyr542 and Tyr580 recruit Grb2/Sos complex to activate Ras-Erk signaling. Recently, it was reported that ZAP70, a non-receptor tyrosine kinase, enhances phosphorylation of Shp2 at Tyr580 and activates Erk. (PMID: 23318428)