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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-POLR2A (Ser5) Recombinant antibody (84834-1-RR)

    WB analysis using 84834-1-RR

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 84834-1-RR (Phospho-POLR2A (Ser5) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-POLR2A (Ser5) Recombinant antibody (84834-1-RR)

    FC experiment of HeLa using 84834-1-RR

    1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-POLR2A (Ser5) Recombinant antibody (84834-1-RR, Clone:241914E5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Phospho-POLR2A (Ser5) Recombinant antibody

Phospho-POLR2A (Ser5) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84834-1-RR
Clone No.241914E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Phospho POLR2A, Phospho POLR2A (Ser5), Phospho-POLR2A, phospho-polymerase II, Phospho-Rpb1 CTD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, λ phosphatase treated HeLa cells
Positive FC (Intra) detected inλ phosphatase treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84834-1-RR targets Phospho-POLR2A (Ser5) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Calculated molecular weight 1970 aa, 217 kDa
Observed molecular weight270 kDa
GenBank accession numberBC137231
Gene Symbol POLR2A
Gene ID (NCBI) 5430
RRIDAB_3672142
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP24928
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA Pol II consists of 12 subunits and is responsible for the expression of mRNA and lincRNA genes within cells. The subunits of RNA polymerases have been shown to play specific roles in Pol II CTD dephosphorylation, tissue-specific transcription, mRNA decay, and translation. Pol II transcription involves initiation through recruitment and CTD serine 5 phosphorylation, elongation with CTD serine 2 phosphorylation, and termination. (PMID: 38842922)

Protocols

Product Specific Protocols
WB protocol for Phospho-POLR2A (Ser5) antibody 84834-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols