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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-POLR2A (Ser5) Recombinant antibody (84834-1-RR)

    WB analysis using 84834-1-RR (same clone as 84834-1-PBS)

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 84834-1-RR (Phospho-POLR2A (Ser5) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84834-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Phospho-POLR2A (Ser5) Recombinant antibody (84834-1-RR)

    FC experiment of HeLa using 84834-1-RR (same clone as 84834-1-PBS)

    1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-POLR2A (Ser5) Recombinant antibody (84834-1-RR, Clone:241914E5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 84834-1-PBS in a different storage buffer formulation.

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Phospho-POLR2A (Ser5) Recombinant antibody, PBS Only

Phospho-POLR2A (Ser5) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84834-1-PBS
Clone No.241914E5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

POLR2A, EC:2.7.7.48, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit A

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84834-1-PBS targets Phospho-POLR2A (Ser5) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Calculated molecular weight 1970 aa, 217 kDa
Observed molecular weight270 kDa
GenBank accession numberBC137231
Gene Symbol POLR2A
Gene ID (NCBI) 5430
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP24928
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

RNA Pol II consists of 12 subunits and is responsible for the expression of mRNA and lincRNA genes within cells. The subunits of RNA polymerases have been shown to play specific roles in Pol II CTD dephosphorylation, tissue-specific transcription, mRNA decay, and translation. Pol II transcription involves initiation through recruitment and CTD serine 5 phosphorylation, elongation with CTD serine 2 phosphorylation, and termination. (PMID: 38842922)