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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-PDPK1 (Ser241) Recombinant antibody (85223-3-RR)

    WB analysis of NIH/3T3 using 85223-3-RR

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85223-3-RR (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using Phospho-PDPK1 (Ser241) Recombinant monoclonal anti (85223-3-RR)

    FC experiment of NIH/3T3 using 85223-3-RR

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Phospho-PDPK1 (Ser241) Recombinant monoclonal antibody (85223-3-RR, Clone:250623A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

Phospho-PDPK1 (Ser241) Recombinant monoclonal antibody

Phospho-PDPK1 (Ser241) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 85223-3-RR
Clone No.250623A11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

p PDPK1, p PDPK1 (Ser241), PDPK1 (Ser241), Phospho PDPK1 (Ser241), Phospho-PDPK1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, λ phosphatase treated NIH/3T3 cells
Positive FC (Intra) detected inλ phosphatase treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85223-3-RR targets Phospho-PDPK1 (Ser241) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name 3-phosphoinositide dependent protein kinase-1
Calculated molecular weight 556 aa, 63 kDa
Observed molecular weight58-68 kDa
GenBank accession numberBC012103
Gene Symbol PDPK1
Gene ID (NCBI) 5170
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO15530
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.

Protocols

Product Specific Protocols
FC protocol for Phospho-PDPK1 (Ser241) antibody 85223-3-RRDownload protocol
WB protocol for Phospho-PDPK1 (Ser241) antibody 85223-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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