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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Phospho-P62/SQSTM1 (Ser349) Recombinant antibody (80294-2-RR)

    WB analysis of HEK-293 using 80294-2-RR (same clone as 80294-2-PBS)

    Non-treated HEK-293 cells and sodium arsenite treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80294-2-RR (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-P62/SQSTM1 (Ser349) Recombinant antibody (80294-2-RR)

    WB analysis of NIH/3T3 using 80294-2-RR (same clone as 80294-2-PBS)

    Non-treated NIH/3T3 cells and MG132 treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80294-2-RR (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80294-2-PBS in a different storage buffer formulation.

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Phospho-P62/SQSTM1 (Ser349) Recombinant antibody, PBS Only

Phospho-P62/SQSTM1 (Ser349) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 80294-2-PBS
Clone No.250277E9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Synonyms

P62, P62/SQSTM1, SQSTM1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80294-2-PBS targets Phospho-P62/SQSTM1 (Ser349) in applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name sequestosome 1
Calculated molecular weight 48 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC017222
Gene Symbol P62/SQSTM1
Gene ID (NCBI) 8878
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13501
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. It functions as a bridge between polyubiquitinated cargo and autophagosomes (PMID:16286508). SQSTM1 is at the cross-roads of several signaling pathways including Keap1-Nrf2 pathway , NFκB pathway , NFE2L2/NRF2 pathway , mTOR pathway and Wnt pathway. Phosphorylation and/or de-phosphorylation of p62-Ser349 may participate in the regulation of both selective autophagy and oxidative stress response (PMID: 33397898).