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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-JNK (Thr183) Recombinant monoclonal antibo (80435-8-RR)

    WB analysis using 80435-8-RR

    Non-treated HSC-T6 cells and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80435-8-RR (Phospho-JNK (Thr183) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of HEK-293 cells using Phospho-JNK (Thr183) Recombinant monoclonal antibo (80435-8-RR)

    WB analysis of HEK-293 using 80435-8-RR

    Non-treated HEK-293 cells and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80435-8-RR (Phospho-JNK (Thr183) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

  • Dot Blot experiment of / using Phospho-JNK (Thr183) Recombinant monoclonal antibo (80435-8-RR)

    Dot Blot experiment of / using 80435-8-RR

    Dot blot analysis was used to confirm the specificity of 80435-8-RR,Phospho-SAPK/JNK (Thr183) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

Phospho-JNK (Thr183) Recombinant monoclonal antibody

Phospho-JNK (Thr183) Uni-rAb® Recombinant Antibody for WB, Dot Blot, ELISA
Cat No. 80435-8-RR
Clone No.253546C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Dot Blot, ELISA

Synonyms

JNK (Thr183), Phospho-JNK, phospho-SAPK, phospho-SAPK (Thr183), Phospho-SAPK/JNK

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inUV treated HEK-293 cells, Calyculin A treated HSC-T6 cells
Positive Dot Blot detected in/ /

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
DOT BLOTDOT BLOT : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80435-8-RR targets Phospho-JNK (Thr183) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name mitogen-activated protein kinase 8
Calculated molecular weight 48 kDa
Observed molecular weight42 kDa, 50 kDa
GenBank accession numberNM_138982
Gene Symbol JNK
Gene ID (NCBI) 5599
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP45983
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. Phospho-JNK (Thr183) is the activated form of c-Jun N-terminal kinase (JNK), a MAPK family serine/threonine kinase. Phosphorylation at Thr183 (often with Tyr185) by MKK4/7 activates JNK, regulating cell apoptosis, proliferation and stress responses. (PMID: 40538844)

Protocols

Product Specific Protocols
WB protocol for Phospho-JNK (Thr183) antibody 80435-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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